Fig. 3: Ultra-rapid-sequencing pipeline performance.
From: Accelerated identification of disease-causing variants with ultra-rapid nanopore genome sequencing
The detailed schema of the ultra-rapid-sequencing pipeline and end-to-end performance of the ultra-rapid-sequencing pipeline on two different clinical samples. a, The ultra-rapid-sequencing pipeline starts from sample collection on the far left to final diagnosis on the far right. The details of each step are presented in Online Methods. All the steps that run in parallel are vertically stacked. b, The first patient was the fourth sample sequenced on the set of flow cells, resulting in 2:16 h of sequencing. Variant calling completed 6:55 h from the start of sample preparation. Subsequently, variant filtration and manual review identified a candidate variant in gene TNNT2 in 7:18 h. c, The second patient was the sixth sample on the same set of flow cells. The sequencing completed in 2:46 h with 200 Gb followed by another 2:44 h to identify a candidate variant resulting in an end-to-end time of 7:48 h.
