Fig. 2: Comparison of barcoded and nonbarcoded variant calling performance, and standard and ultra-rapid variant filtration performance.
From: Accelerated identification of disease-causing variants with ultra-rapid nanopore genome sequencing
a, Stratified variant calling performance comparison between barcoded and nonbarcoded HG002 samples in all benchmarking and exonic regions. The HG002 nonbarcoded run was the seventh sample run on the flow cells. The similarity in variant calling performance shows that barcoding is not necessary to achieve high-quality variant calls. b, Comparison between standard and ultra-rapid variant filtration pipelines. The standard pipeline picks variants if any of the subcategories are true for any variant, whereas the ultra-rapid pipeline uses a score-based method to surface mostly relevant variants for fast manual review. We see that the standard pipeline flags 147 variants compared with 31 variants proposed by the ultra-rapid pipeline, which substantially reduces the time required for manual review.
