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The expressions ''most significant bit first'' and ''least significant bit at last'' are indications on the ordering of the sequence of the bits in the bytes sent over a wire in a [[serial transmission]] protocol or in a stream (e.g. an audio stream).
''Most significant bit first'' means that the most
''Least significant bit first'' means that the [[least significant bit]] will arrive first: hence e.g. the same hexadecimal number <code>0x12</code>, again <code>00010010</code> in binary representation, will arrive as the (reversed) sequence <code>0 1 0 0 1 0 0 0</code>.
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